Composite

Part:BBa_K3352004:Design

Designed by: Hannah Hsu   Group: iGEM20_TAS_Taipei   (2020-10-18)


Strong Promoter and RBS SplintR Ligase Expressing Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 851
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added a His-Tag to our proteins so that we could perform a His-purification on it.


Source

Paramecium bursaria Chlorella virus 1.

References