Composite
Part:BBa_K3352004:Design
Designed by: Hannah Hsu Group: iGEM20_TAS_Taipei (2020-10-18)
Strong Promoter and RBS SplintR Ligase Expressing Construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 851
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added a His-Tag to our proteins so that we could perform a His-purification on it.
Source
Paramecium bursaria Chlorella virus 1.